Synonyms
CD121b antibody, IL1RB antibody, MGC47725 antibody, IL1R2 antibody, IL-1RT2 antibody, antigen CDw121b antibody, interleukin-1 receptor beta antibody, CD121 antigen-like family member B antibody, IL-1R-beta antibody, IL-1R-2 antibody, interleukin-1 receptor type II antibody, interleukin-1 receptor type 2 antibody, "type II interleukin-1 receptor, beta antibody", IL-1RT-2 antibody, CDw121b antibody, "interleukin 1 receptor, type II antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
45 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 172 and 398 of IL1 Receptor 2 (Uniprot ID# P27930)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A549, using IL1 Receptor 2(107-10792) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using IL1R2(107-10792) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
107-10792 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.