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Anti-IL10 (Center) Antibody
Catalog #: 102-18146
Rabbit Anti-Human IL10 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P22301 |
| Gene Id | 3586 |
| Gene Symbols | IL10 |
| Protein Name / Synonyms | IL10 (Center) |
| Clonality | Polyclonal |
| Preparation | This IL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-53 amino acids from the Central region of human IL10. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Specificity
Predicted Reactivity
EBV, Monkey
Other Names
IL10; Interleukin-10; Cytokine synthesis inhibitory factor
NCBI Accession #
NP_000563.1
Other Accession #
P79338; P03180
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
20517
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of IL10 Antibody (Center) (Cat# 102-18146) in MDA-MB435 cell line lysates (35ug/lane). IL10 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with IL10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | IL10 Antibody (Center) (Cat# 102-18146) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.