Anti-IL4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P05112
Gene Id	3565
Gene Symbols	IL4
Protein Name / Synonyms	IL4 (C-term)
Clonality	Polyclonal
Preparation	This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.

Specificity

Predicted Reactivity

Monkey

Other Names

IL4; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

NCBI Accession #

NP_000580.1;NP_758858.1

Other Accession #

P79339

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

17492

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	(LEFT)Western blot analysis of IL4 Antibody (C-term) (Cat# 102-16447) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) (Cat# 102-16447) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.
	IL4 Antibody (C-term) (Cat# 102-16447) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	IL4 Antibody (C-term) (Cat# 102-16447) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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