Anti-IL5RA (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q01344
Gene Id	3568
Gene Symbols	IL5RA
Protein Name / Synonyms	IL5RA (N-term)
Clonality	Polyclonal
Preparation	This IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human IL5RA.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.

Specificity

Other Names

IL5RA; IL5R; Interleukin-5 receptor subunit alpha; CDw125; CD_antigen=CD125; Flags: Precursor

NCBI Accession #

NP_000555.2;NP_001230028.1;NP_783851.1;NP_783852.1;NP_783853.1;NP_783854.1;NP_783855.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

47685

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody (N-term)(Cat# 102-16445). 102-16445 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	IL5RA Antibody (N-term) (Cat# 102-16445) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL5RA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	IL5RA Antibody (N-term) (Cat# 102-16445) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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