Anti-IL8 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P10145
Gene Id	3576
Gene Symbols	IL8
Protein Name / Synonyms	IL8 (C-term)
Clonality	Polyclonal
Preparation	This IL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-99 amino acids from the C-terminal region of human IL8.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor.

Specificity

Other Names

IL8; CXCL8; Interleukin-8; C-X-C motif chemokine 8; Emoctakin; Granulocyte chemotactic protein 1; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; Neutrophil-activating protein 1; Protein 3-10C; T-cell chemotactic factor; MDNCF-a; GCP/IL-8 protein IV; IL8/NAP1 form I; (Ala-IL-8)77; GCP/IL-8 protein II; IL-8(1-77); IL8/NAP1 form II; MDNCF-b; IL-8(5-77); IL-8(6-77); (Ser-IL-8)72; GCP/IL-8 protein I; IL8/NAP1 form III; Lymphocyte-derived neutrophil-activating factor; MDNCF-c; Neutrophil-activating factor; IL-8(7-77); GCP/IL-8 protein V; IL8/NAP1 form IV; IL-8(8-77); GCP/IL-8 protein VI; IL8/NAP1 form V; IL-8(9-77); GCP/IL-8 protein III; IL8/NAP1 form VI

NCBI Accession #

NP_000575.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11098

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of IL8 Antibody (C-term) (Cat# 102-17944) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of IL8 Antibody (C-term)(Cat# 102-17944) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	IL8 Antibody (C-term) (Cat# 102-17944) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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