Anti-ILK1/ILK2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O55222
Gene Id	16202
Gene Symbols	ILK
Protein Name / Synonyms	ILK1/ILK2 (N-term)
Clonality	Polyclonal
Preparation	This ILK1/ILK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 11-41 amino acids from the N-terminal region of human ILK1/ILK2.
Concentration (lot specific)	1.430 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK encodes a predicted 451-amino acid protein, with an apparent molecular weight of 59 kD. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

Ilk; Integrin-linked protein kinase

NCBI Accession #

NP_001155196.1;NP_034692.2

Other Accession #

Q99J82; Q13418; Q3SWY2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

51373

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-ILK1 Pab (Cat# 102-17517) in HL60 cell lysate. ILK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK1/ILK2 Antibody (N-term) (Cat# 102-17517), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

MOUSE

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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