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Anti-Importin 13 (C-term) Antibody
Catalog #: 107-10722
Importin 13 antibody [C3], C-Terminus, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 9670 |
| Protein Name / Synonyms | Importin 13, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
IMP13 antibody, KAP13 antibody, KIAA0724 antibody, LGL2 antibody, RANBP13 antibody, IPO13 antibody, ran-binding protein 13 antibody, karyopherin 13 antibody, importin-13 antibody, karyopherin-13 antibody, late gestation lung 2 antibody, Ran binding protein 13 antibody, importin 13 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
108 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 901 and 963 of Importin 13 (Uniprot ID# O94829)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 10 µg / 1mg whole cell lysate. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using Importin 13(107-10722) antibody at 1:100 dilution.
Importin 13 antibody [C3], C-term detects Importin 13 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
Importin 13 antibody [C3], C-term (107-10722) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
Importin 13 antibody [C3], C-term (107-10722) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Importin 13(107-10722) antibody at 1:200 dilution.
IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody (107-10722).
The precipitated IPO13 was detected by 107-10722 diluted at 1:1000.
The precipitated IPO13 was detected by 107-10722 diluted at 1:1000.
Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody.
A: Non-transfected lysate.
B: IPO13 transfected lysate.
7.5% SDS PAGE
107-10722 diluted at 1:500
A: Non-transfected lysate.
B: IPO13 transfected lysate.
7.5% SDS PAGE
107-10722 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.