Anti-INHA (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P05111
Gene Id	3623
Gene Symbols	INHA
Protein Name / Synonyms	INHA (N-term)
Clonality	Polyclonal
Preparation	This INHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human INHA.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, IHC-P, Western Blotting, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.

Specificity

Other Names

INHA; Inhibin alpha chain

NCBI Accession #

NP_002182.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39670

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of NHA Antibody (N-term)(Cat# 102-16910) in mouse testis tissue lysates (35ug/lane).NHA (arrow) was detected using the purified Pab.
	Western blot analysis of INHA(arrow) using rabbit polyclonal INHA Antibody (N-term)(Cat# 102-16910). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INHA gene (Lane 2) (Origene Technologies).
	INHA Antibody (N-term) (Cat# 102-16910) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INHA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of INHA Antibody (N-term)(Cat# 102-16910) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	INHA Antibody (N-term) (Cat# 102-16910) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.