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Anti-INSIG1 (N-term) Antibody
Catalog #: 102-18086
Rabbit Anti-Human INSIG1 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O15503 |
| Gene Id | 3638 |
| Gene Symbols | INSIG1 |
| Protein Name / Synonyms | INSIG1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This INSIG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human INSIG1. |
| Concentration (lot specific) | 0.440 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
INSIG1 is an insulin-induced protein. This protein encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.
Specificity
Other Names
INSIG1; Insulin-induced gene 1 protein
NCBI Accession #
NP_005533.2;NP_938150.2;NP_938151.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29987
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
 | Western blot analysis of INSIG1 Antibody (N-term) (Cat# 102-18086) in Hela cell line lysates (35ug/lane). INSIG1 (arrow) was detected using the purified Pab. |
 | INSIG1 Antibody (N-term) (Cat# 102-18086) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | INSIG1 Antibody (N-term) (Cat# 102-18086) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INSIG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.