Synonyms
IFN-alphaA antibody, IFNA antibody, INFA2 antibody, MGC125764 antibody, MGC125765 antibody, IFNA2 antibody, alpha-2a interferon antibody, interferon alpha 2b antibody, IFN-alpha-2 antibody, leIF A antibody, interferon alpha A antibody, interferon alpha-2 antibody, "interferon, alpha 2 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 125 and 188 of Interferon alpha 2 (Uniprot ID# P01563)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using interferon alpha 2(107-10751) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using interferon alpha 2(107-10751) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10751 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.