Anti-Interferon-inducible protein (IFITM3) (N-term) Antibody

Catalog #: 102-11135

Rabbit Anti-Human Interferon-inducible protein (IFITM3) (N-term) Antibody, 400 ul

Specifications

Description

Introduction

The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.

Specificity

Other Names

IFITM3; Interferon-induced transmembrane protein 3; Dispanin subfamily A member 2b; Interferon-inducible protein 1-8U

NCBI Accession #

NP_066362.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14632

Recommended Dilutions

WB: 1:1000; IF: 1:100-500

	Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term)(Cat# 102-11135).102-11135 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
	IFITM3 Antibody (N-term) (Cat# 102-11135) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).
	Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.