3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-IP6K1 Antibody
Catalog #: 107-10417
IP6K1 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 9807 |
| Protein Name / Synonyms | IP6K1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
IHPK1 antibody, MGC9925 antibody, PiUS antibody, IP6K1 antibody, insP6 kinase 1 antibody, inositol hexakisphosphate kinase 1 antibody, ATP:1D-myo-inositol-hexakisphosphate phosphotransferase antibody, Pi uptake stimulator antibody, inositol hexaphosphate kinase 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 50 and 309 of IP6K1 (Uniprot ID# Q92551)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1(107-10417) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
IP6K1 antibody detects IP6K1 protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg THP-1 whole cell lysate/extract
D. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
IP6K1 antibody (107-10417) dilution: 1:1000
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg THP-1 whole cell lysate/extract
D. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
IP6K1 antibody (107-10417) dilution: 1:1000
IP6K1 antibody detects IP6K1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human IHPK1-transfected 293T cells
10 % SDS-PAGE
IP6K1 antibody (107-10417) dilution: 1:5000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human IHPK1-transfected 293T cells
10 % SDS-PAGE
IP6K1 antibody (107-10417) dilution: 1:5000
Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1(107-10417) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.