Anti-IRAK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P51617
Gene Id	3654
Gene Symbols	IRAK1
Protein Name / Synonyms	IRAK (C-term)
Clonality	Polyclonal
Preparation	This IRAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 683-712 amino acids from the C-terminal region of human IRAK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

Specificity

Other Names

IRAK1; IRAK; Interleukin-1 receptor-associated kinase 1

NCBI Accession #

NP_001020413.1;NP_001020414.1;NP_001560.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

76537

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-IRAK1 Pab (Cat# 102-17630) in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
	Western blot analysis of IRAK1 (arrow) using rabbit polyclonal IRAK Antibody (C-term) (Cat#AP802b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human Placenta tissue reacted with IRAK Antibody (C-term)(Cat# 102-17630), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human Placenta tissue reacted with IRAK Antibody (C-term)(Cat# 102-17630), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.