Anti-JAM-B Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	58494
Protein Name / Synonyms	JAM-B
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C21orf43 antibody, CD322 antibody, JAM-B antibody, JAMB antibody, PRO245 antibody, VE-JAM antibody, VEJAM antibody, JAM2 antibody, JAM-IT/VE-JAM antibody, JAM-2 antibody, junctional adhesion molecule B antibody, vascular endothelial junction-associated molecule antibody, junctional adhesion molecule 2 antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

33 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 294 of JAM-B (Uniprot ID# P57087)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded TOV-112D xenograft, using JAM-B(107-10457) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded mouse small intestine, using JAM-B(107-10457) antibody at 1:200 dilution.(Image courtesy of Koji Taniguchi, Ph.D (Laboratory of Dr. Michael Karin, UCSD.)

Immunohistochemical analysis of paraffin-embedded human colon, using JAM-B(107-10457) antibody at 1:200 dilution.(Image courtesy of Sergei Grivennikov Ph.D (Laboratory of Dr. Michael Karin, UCSD.)

JAM-B antibody detects JAM2 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
JAM-B antibody (107-10457) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: A431
12 % SDS PAGE
107-10457 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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