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Anti-JHDM2b (Center) Antibody
Catalog #: 102-11382
Rabbit Anti-Human JHDM2b (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q7LBC6 |
| Gene Id | 51780 |
| Gene Symbols | KDM3B |
| Protein Name / Synonyms | JHDM2b (Center) |
| Clonality | Polyclonal |
| Preparation | This JHDM2b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 869-897 amino acids from the Central region of human JHDM2b. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Specificity
Predicted Reactivity
Mouse
Other Names
KDM3B; C5orf7; JHDM2B; JMJD1B; KIAA1082; Lysine-specific demethylase 3B; JmjC domain-containing histone demethylation protein 2B; Jumonji domain-containing protein 1B; Nuclear protein 5qNCA
NCBI Accession #
NP_057688.2
Other Accession #
Q6ZPY7; NP_057688.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
191581
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | JHDM2b Antibody (Center) (Cat# 102-11382) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the JHDM2b antibody detected the JHDM2b protein (arrow). |
 | JHDM2b Antibody (Center) (Cat# 102-11382) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.