Anti-JMJD3 (N-term) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O15054
Gene Id	23135
Gene Symbols	KDM6B
Protein Name / Synonyms	JMJD3 (N-term)
Clonality	Polyclonal
Preparation	This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Specificity

Predicted Reactivity

Mouse

Other Names

KDM6B; JMJD3; KIAA0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B

NCBI Accession #

NP_001073893.1

Other Accession #

Q5NCY0

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

176632

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of anti-JMJD3 (N-term) Pab (102-10599) in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term) (Cat# 102-10599), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) (Cat# 102-10599) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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