Anti-KHS (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	11183
Protein Name / Synonyms	KHS, Internal
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

GCKR antibody, KHS antibody, KHS1 antibody, MAPKKKK5 antibody, MAP4K5 antibody, MEK kinase kinase 5 antibody, kinase homologous to SPS1/STE20 antibody, MEKKK 5 antibody, MAPK/ERK kinase kinase kinase 5 antibody, germinal center kinase-related antibody, mitogen-activated protein kinase kinase kinase kinase 5 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

95 kDa

Immunogen

Synthetic peptide contain a sequence corresponding to a region within amino acids 417 and 482 of KHS

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using KHS(107-10832) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KHS(107-10832) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
MAP4K5 antibody
107-10832 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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