Anti-KLF4 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O43474
Gene Id	9314
Gene Symbols	KLF4
Protein Name / Synonyms	KLF4
Clonality	Polyclonal
Preparation	This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-504 amino acids from human KLF4.
Concentration (lot specific)	0.420 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Angiogenesis, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

KLF4; EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor

NCBI Accession #

NP_004226.3

Other Accession #

Q60793; Q9ET58; Q60843; Q9Y5W3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54671

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

	Immunofluorescent analysis of U251 cells, using KLF4 Antibody (Cat# 102-15099). 102-15099 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor^® 554 (red) conjugated Phalloidin (red).
	Western blot analysis of KLF4-S457 (Cat# 102-15099) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.
	Fluorescent confocal image of HeLa cells stained with 102-15099 KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15099 KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.
	Fluorescent confocal image of SY5Y cells stained with 102-15099 KLF4 (S457) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15099 KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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