Anti-KLF5 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13887
Gene Id	688
Gene Symbols	KLF5
Protein Name / Synonyms	KLF5 (C-term)
Clonality	Polyclonal
Preparation	This KLF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human KLF5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.

Specificity

Predicted Reactivity

Mouse

Other Names

KLF5; BTEB2; CKLF; IKLF; Krueppel-like factor 5; Basic transcription element-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2

NCBI Accession #

NP_001721.2

Other Accession #

Q9Z0Z7

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

50792

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50; FC: 1:10-50

	KLF5 Antibody (C-term) (Cat# 102-17291) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).
	Fluorescent confocal image of U251 cell stained with KLF5 Antibody (C-term)(Cat# 102-17291).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
	KLF5 Antibody (C-term) (Cat# 102-17291) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	KLF5 Antibody (C-term) (Cat# 102-17291) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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