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Anti-KLH Antibody, Biotinylated
Catalog #: RB-14-0006B-50
Mouse anti-KLH Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Limpet |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Immunogen | Immunogen was KLH carrier protein. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. The identified clone was called as 11E8F5C3. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells derived from the immunized mouse. The IgM fraction of cell culture supernatant was purified by affinity chromatography. |
| Isotype | IgM |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000-5, 000), Western Blotting( recommended work dilution= 2-5 µg/ml) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The most commonly used carrier proteins are KLH (Keyhole Limpet Hemocyanin), BSA (Bovine Serum Albumin) and OVA (Ovalbumin). These carrier proteins have different properties. KLH is the largest, heterogeneous glycosylated protein consisting of subunits. The largest has a molecular weight (MW) in the range 4.5 x 105 - 1.3 107 .
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The mouse anti-KLH antibody detects individual KLH and conjugated protein, as well as KLHconjugated peptide in both ELISA and Western Blot.
References
- H¶glund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.
Expiration:
12 months from the date of shipment when stored properly.