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Anti-KPNA4 Antibody
Catalog #: 144-02026
KPNA4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00629 |
| Gene Id | 3840 |
| Gene Symbols | KPNA4 |
| Protein Name / Synonyms | KPNA4, IPOA3, QIP1, SRP3, importin subunit alpha-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
57kDa
Observed MW
58kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA4 (NP_002259.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using KPNA4 antibody (144-02026) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using KPNA4 Antibody (144-02026) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using KPNA4 Antibody (144-02026) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KPNA4 Antibody (144-02026) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody (144-02026) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using KPNA4 Antibody (144-02026) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colonic muscle using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using KPNA4 Antibody (144-02026) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using KPNA4 antibody (144-02026). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.