KPNA4 antibody [C3], C-Terminus, 0.73 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3840 |
| Protein Name / Synonyms | KPNA4, C-term |
| Concentration (lot specific) | 0.73 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
IPOA3 antibody, MGC12217 antibody, MGC26703 antibody, QIP1 antibody, SRP3 antibody, KPNA4 antibody, importin alpha Q1 antibody, importin subunit alpha-4 antibody, karyopherin subunit alpha-4 antibody, karyopherin alpha 4 (importin alpha 3) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
58 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 459 and 521 of KPNA4 (Uniprot ID# O00629)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (107-10607) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (107-10607) dilution: 1:1000
KPNA4 antibody [C3], C-term detects KPNA4 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (107-10607) dilution: 1:1000
A. 30 µg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
KPNA4 antibody [C3], C-term (107-10607) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (107-10607) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4(107-10607) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: HepG2
C: Molt-4
7.5% SDS PAGE
107-10607 diluted at 1:1500
A: 293T
B: HepG2
C: Molt-4
7.5% SDS PAGE
107-10607 diluted at 1:1500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.