Synonyms
HRB2 antibody, RIP-1 antibody, KRR1 antibody, HIV-1 Rev binding protein 2 antibody, rev-interacting protein 1 antibody, KRR1 small subunit processome component homolog antibody, HIV-1 Rev-binding protein 2 antibody, Rev interacting protein antibody, "KRR1, small subunit (SSU) processome component, homolog (yeast) antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 47 and 344 of KRR1 (Uniprot ID# Q13601)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using KRR1(107-10558) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using KRR1(107-10558) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
10% SDS PAGE
107-10558 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.