Anti-Ku70 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P12956
Gene Id	2547
Gene Symbols	XRCC6
Protein Name / Synonyms	Ku70 (Center)
Clonality	Polyclonal
Preparation	This Ku70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the Central region of human Ku70.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Ku70 is a single stranded DNA-dependent ATP-dependent helicase. It has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.

Specificity

Other Names

XRCC6; G22P1; X-ray repair cross-complementing protein 6; 5'-deoxyribose-5-phosphate lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 6

NCBI Accession #

NP_001460.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

69843

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of Ku70 (arrow) using rabbit polyclonal Ku70 Antibody (Center) (Cat# 102-16930). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ku70 gene (Lane 2).
	Formalin-fixed and paraffin-embedded mouse spleen tissue reacted with Ku70 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Ku70 Antibody (Center) (Cat# 102-16930) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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