RayBiotech

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Anti-LAIR1 Antibody

Catalog #: 130-10471

Mouse anti-LAIR1 (5B1-E6-G2-G1-G8) Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Accession Number
Q6GTX8
Gene Id
3903
Gene Symbols
LAIR1 CD305
Protein Name / Synonyms
Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) (hLAIR1) (CD antigen CD305)
Clone5B1-E6-G2-G1-G8
Immunogen
Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Recommended ApplicationsELISA (recommended work dilution= 1:256, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000),
Antigen Catalog Number
230-00599
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Leukocyte-associated immunoglobulin-like receptor 1 is a protein that in humans is encoded by theLAIR1 gene.The protein is an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Immunohistochemistry (IHC) Analysis

IHC revealed positive staining in human colon tissue. The Rabbit Anti-Human LAIR1 antibody (130-10471) was used at a 200-fold dilution, followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, and counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer at pH9.0 before initiating the IHC staining (40X).

Western Blot Analysis

Primary Antibody: Mouse Anti-LAIR1 (Cat# 130-10471) 1:1000
Secondary Antibody: HRP Goat Anti-Mouse IgG (H + L) 1:5000

Storage/Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.