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Anti-LAIR1 Antibody, Biotinylated

Catalog #: 130-10471B-50

Mouse anti-LAIR1 (5B1-E6-G2-G1-G8) Biotinylated Antibody, 50 ug

Product Description

Specifications

Size50 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesMouse
Accession Number
Q6GTX8
Gene Id
3903
Gene Symbols
LAIR1 CD305
Protein Name / Synonyms
Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) (hLAIR1) (CD antigen CD305)
ClonalityMonoclonal
Clone5B1-E6-G2-G1-G8
Immunogen
Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
IsotypeIgG1
Recommended ApplicationsELISA (recommended work dilution= 1:256, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000),
Reconstitution
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Leukocyte-associated immunoglobulin-like receptor 1 is a protein that in humans is encoded by theLAIR1 gene.The protein is an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Immunohistochemistry (IHC) Analysis

IHC revealed positive staining in human colon tissue. The Rabbit Anti-Human LAIR1 antibody (130-10471) was used at a 200-fold dilution, followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, and counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer at pH9.0 before initiating the IHC staining (40X).

Western Blot Analysis

Primary Antibody: Mouse Anti-LAIR1 (Cat# 130-10471) 1:1000
Secondary Antibody: HRP Goat Anti-Mouse IgG (H + L) 1:5000

Storage/Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.