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Anti-Lamin A/C Antibody
Catalog #: 107-10980
Lamin A/C antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4000 |
| Protein Name / Synonyms | Lamin A/C |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CDCD1 antibody, CDDC antibody, CMD1A antibody, CMT2B1 antibody, EMD2 antibody, FPL antibody, FPLD antibody, HGPS antibody, IDC antibody, LDP1 antibody, LFP antibody, LGMD1B antibody, LMN1 antibody, LMNC antibody, LMNL1 antibody, PRO1 antibody, LMNA antibody, renal carcinoma antigen NY-REN-32 antibody, lamin A/C-like 1 antibody, prelamin-A/C antibody, 70 kDa lamin antibody, lamin A/C antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
74 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 59 and 249 (P02545) of Lamin A/C
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Lamin A/C antibody detects LMNA protein by Western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin A/C antibody (107-10980) dilution: 1:1000
A. 30 µg NIH-3T3 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin A/C antibody (107-10980) dilution: 1:1000
Sample (20 ug )
A: HeLa Nucleus
10% SDS PAGE
107-10980 diluted at 1:3000
A: HeLa Nucleus
10% SDS PAGE
107-10980 diluted at 1:3000
Immunohistochemical analysis of paraffin-embedded H441 Xenograft, using Lamin A/C(107-10980) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.