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Anti-Lamin B2 (internal) Antibody
Catalog #: 107-10877
Lamin B2 antibody [N3C2], Internal, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 84823 |
| Protein Name / Synonyms | Lamin B2, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
LAMB2 antibody, LMN2 antibody, MGC2721 antibody, LMNB2 antibody, lamin-B2 antibody, lamin B2 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 221 and 500 of Lamin B2 (Uniprot ID# Q03252)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein at on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500.
Sample: Paraffin-embedded Cal27 xenograft.
Lamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500.
Lamin B2 antibody [N3C2], Internal immunoprecipitates Lamin B2 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of Lamin B2 protein expressing HepG2 cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of Lamin B2 by 3 µg of Lamin B2 antibody [N3C2], Internal (107-10877) 7.5% SDS-PAGE The immunoprecipitated Lamin B2 protein was detected by Lamin B2 antibody [N3C2], Internal (107-10877) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Lamin B2 antibody [N3C2], Internal detects LMNB2 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
7.5 % SDS-PAGE
Lamin B2 antibody [N3C2], Internal (107-10877) dilution: 1:500
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
7.5% SDS PAGE
107-10877 diluted at 1:1000
A: H1299
B: Hela
C: Hep G2
7.5% SDS PAGE
107-10877 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.