Anti-Laminin gamma 2 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	3918
Protein Name / Synonyms	Laminin gamma 2, Internal
Concentration (lot specific)	0.66 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Cell Adhesion
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

B2T antibody, BM600 antibody, CSF antibody, EBR2 antibody, EBR2A antibody, LAMB2T antibody, LAMNB2 antibody, MGC138491 antibody, MGC141938 antibody, LAMC2 antibody, nicein subunit gamma antibody, kalinin subunit gamma antibody, CSF 140 kDa subunit antibody, BM600-100kDa antibody, laminin B2t chain antibody, laminin subunit gamma-2 antibody, large adhesive scatter factor 140 kDa subunit antibody, epiligrin subunit gamma antibody, cell-scattering factor 140 kDa subunit antibody, ladsin 140 kDa subunit antibody, laminin, gamma 2 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

131 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 445 and 657 of Laminin gamma 2 (Uniprot ID# Q13753)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using Laminin gamma 2(107-11091) antibody at 1:250 dilution.

Laminin gamma 2 antibody detects Laminin gamma 2 protein by Western blot analysis.
A. 30 µg Raji whole cell lysate/extract
B. 30 µg NCI-H929 whole cell lysate/extract
5 % SDS-PAGE
Laminin gamma 2 antibody (107-11091) dilution: 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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