Anti-LCK (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P06239
Gene Id	3932
Gene Symbols	LCK
Protein Name / Synonyms	LCK (C-term)
Clonality	Polyclonal
Preparation	This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-509 amino acids from the C-terminal region of human LCK.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.

Specificity

Other Names

LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK

NCBI Accession #

NP_001036236.1;NP_005347.3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58001

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	LCK Antibody (C-term) (Cat# 102-15169) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow).
	Formalin-fixed and paraffin-embedded human lymphoma reacted with LCK Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	LCK Antibody (C-term) (Cat# 102-15169) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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