Anti-LCK (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P06239
Gene Id	3932
Gene Symbols	LCK
Protein Name / Synonyms	LCK (N-term)
Clonality	Polyclonal
Preparation	This LCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human LCK.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

LSK, which belongs to the SRC subfamily of Tyr protein kinases, may participate in antigen-induced T-cell activation. It binds to phosphatidylinositol 3'-kinase (PI3K) from T lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. LSK is bound to the cytoplasmic domain of either CD4 or CD8. This protein is involved in leukemias by a chromosomal translocation t(1;7)(p34;q34) which involves LCK and T-cell receptor beta chain (TCRB) genes.

Specificity

Other Names

LCK; Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK

NCBI Accession #

NP_001036236.1;NP_005347.3

Other Accession #

P06240

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58001

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Confocal immunofluorescent analysis of LCK Antibody (N-term)(Cat# 102-17579) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	LCK Antibody (N-term) (Cat# 102-17579) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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