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Anti-Leptin Receptor (LEPR) (N-term) Antibody
Catalog #: 102-16658
Rabbit Anti-Human Leptin Receptor (LEPR) (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48357 |
| Gene Id | 54741 |
| Gene Symbols | LEPR |
| Protein Name / Synonyms | Leptin Receptor (LEPR) (N-term) |
| Clonality | Polyclonal |
| Preparation | This Leptin Receptor (LEPR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human Leptin Receptor (LEPR). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P |
| Research Area | Angiogenesis, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR). LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.
Specificity
Other Names
LEPR; DB; OBR; Leptin receptor; HuB219; OB receptor; CD_antigen=CD295; Flags: Precursor
NCBI Accession #
NP_001003679.1;NP_001003680.1;NP_001185616.1;NP_001185617.1;NP_001185618.1;NP_002294.2
Other Accession #
NP_002294
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
132494
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
 | Leptin Receptor (LEPR) Antibody (N-term)(Cat# 102-16658) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow). |
 | Leptin Receptor (LEPR) Antibody (N-term) (Cat# 102-16658) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.