Anti-LIM Kinase 1 (LIMK1) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P53667
Gene Id	3984
Gene Symbols	LIMK1
Protein Name / Synonyms	LIM Kinase 1 (LIMK1) (N-term)
Clonality	Polyclonal
Preparation	This LIM Kinase 1 (LIMK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIM Kinase 1 (LIMK1).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IHC-P, Western Blotting
Research Area	Phosphorylation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

LIMK1, a member of the Ser/Thr protein kinase family, may be a component of an intracellular signaling pathway and may be involved in brain development. It phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1, and this cytoplasmic protein also binds ROCK1, whic phosphorylates LIMK1 on serine and/or threonine residues. Highest expression occurs in both adult and fetal nervous systems. It is detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex, and is expressed to a lesser extent in heart and skeletal muscle. Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23. This protein contains 2 LIM zinc-binding domains and 1 PDZ/DHR domain.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

LIMK1; LIMK; LIM domain kinase 1

NCBI Accession #

NP_001191355.1;NP_002305.1

Other Accession #

P53669; P53668

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

72585

Recommended Dilutions

IHC: 1:50-100; WB: 1:1000

	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	LIMK1 Antibody (T9) (Cat# 102-17640) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the LIMK1 antibody detected the LIMK1 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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