Anti-LIMCH1 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	22998
Protein Name / Synonyms	LIMCH1, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp434I0312 antibody, DKFZp686A01247 antibody, DKFZp686B2470 antibody, DKFZp686G18243 antibody, DKFZp686G2094 antibody, DKFZp781C1754 antibody, DKFZp781I1455 antibody, LIMCH1A antibody, LMO7B antibody, MGC72127 antibody, LIMCH1 antibody, LIM and calponin homology domains-containing protein 1 antibody, LIM and calponin homology domains 1 antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

122 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 734 and 1007 (Q9UPQ0) of LIMCH1

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LIMCH1(107-10789) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10789 diluted at 1:2000

Sample (30µg whole cell lysate)
A:H1299
7.5% SDS PAGE
107-10789 diluted at 1:3000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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