Anti-LIN28B (Center) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6ZN17
Gene Id	389421
Gene Symbols	LIN28B
Protein Name / Synonyms	LIN28B (Center)
Clonality	Polyclonal
Preparation	This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids from the Central region of human LIN28B.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Specificity

Predicted Reactivity

Mouse

Other Names

LIN28B; CSDD2; Protein lin-28 homolog B

NCBI Accession #

NP_001004317.1

Other Accession #

Q45KJ6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27084

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of anti-LIN28B Pab (Cat# 102-12694) in K562 cell line lysates (35ug/lane). LIN28B(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human testis tissue reacted with LIN28B antibody (Center) (Cat# 102-12694), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Formalin-fixed and paraffin-embedded human Spleen tissue reacted with LIN28B antibody (Center) (Cat# 102-12694), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HL-60 cells using LIN28B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of LIN28B Antibody (Center) (Cat# 102-12694) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).