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Anti-Livin Antibody, Biotinylated
Catalog #: RB-02-0028B-50
Rabbit anti-Livin Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96CA5 |
| Gene Id | 79444 |
| Gene Symbols | BIRC7 KIAP LIVIN MLIAP RNF50 UNQ5800/PRO19607/PRO21344 |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide (Livin). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography. |
| Immunogen Sequence | CRAPVRSRVRTFLS |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Livin is a member of the inhibitors of apoptosis (IAP) family, which plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Livin (BIRC7/ML-IAP) contains a single BIR domain and RING finger motif that is highly expressed in human melanomas and absent in normal tissues. It is localized in the nucleus and diffusely in the cytoplasm. The livin gene encodes two splicing variants, a 298 amino acid isoform (alpha) and a 280 amino acid form (beta), with different biological activity to various apoptotic stimuli. In addition to directly inhibiting caspase activity, livin can promote the degradation of the proapoptotic protein Smac through the ubiquitin-proteasome pathway.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human Livin. The cross reactivity with mouse and rat Livin was not tested.
References
- B., Yan; (2011) Research progress on Livin protein: an inhibitor of apoptosis. Mol Cell Biochem. 357(1-2):39-45.
- Kasof GM, Gomes BC. (2001) Livin, a novel inhibitor of apoptosis protein family member. J Biol Chem. 276(5):3238-46.
Expiration:
12 months from the date of shipment when stored properly.