LRP130 antibody [N3C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10128 |
| Protein Name / Synonyms | LRP130, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CLONE-23970 antibody, GP130 antibody, LRP130 antibody, LSFC antibody, LRPPRC antibody, "leucine-rich PPR motif-containing protein, mitochondrial antibody", 130 kDa leucine-rich protein antibody, mitochondrial leucine-rich PPR motif-containing protein antibody, LRP 130 antibody, leucine-rich PPR-motif containing antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
158 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 611 and 937 of LRP130 (Uniprot ID# P42704)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
LRP130 antibody [N3C2], Internal detects LRP130 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (107-10849) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: LRP130 protein stained by LRP130 antibody [N3C2], Internal (107-10849) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10849 diluted at 1:5000
A: Mouse brain
5% SDS PAGE
107-10849 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using LRP130 (107-10849) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: HeLa
5% SDS PAGE
107-10849 diluted at 1:1000
A: H1299
B: HeLa
5% SDS PAGE
107-10849 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.