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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 84232 |
| Protein Name / Synonyms | MAF1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC20332 antibody, MGC31779 antibody, MGC39758 antibody, MAF1 antibody, repressor of RNA polymerase III transcription MAF1 homolog antibody, homolog of yeast MAF1 antibody, MAF1 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 201 of MAF1 (Uniprot ID# Q9H063)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MAF1 antibody detects MAF1 protein at nucleus and cytoplasm on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
MAF1 antibody (107-10643) dilution: 1:500.
Sample: Paraffin-embedded colon carcinoma.
MAF1 antibody (107-10643) dilution: 1:500.
MAF1 antibody detects MAF1 protein by Western blot analysis.
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
12 % SDS-PAGE
MAF1 antibody (107-10643) dilution: 1:1000
A. 30 µg GL261 whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
12 % SDS-PAGE
MAF1 antibody (107-10643) dilution: 1:1000
MAF1 antibody detects MAF1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12 % SDS-PAGE
MAF1 antibody (107-10643) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
12 % SDS-PAGE
MAF1 antibody (107-10643) dilution: 1:1000
MAF1 antibody detects MAF1 protein at nucleus on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
MAF1 antibody (107-10643) dilution: 1:500.
Sample: Paraffin-embedded human endometrial carcinoma.
MAF1 antibody (107-10643) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MAF1(107-10643) antibody at 1:200 dilution.
Sample(30 ug whole cell lysate)
A:A431
B:MOLT4
12% SDS PAGE
107-10643 diluted at 1:1000
A:A431
B:MOLT4
12% SDS PAGE
107-10643 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
