Anti-MAGEA11 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	4110
Protein Name / Synonyms	MAGEA11
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CT1.11 antibody, MAGE-11 antibody, MAGE11 antibody, MAGEA-11 antibody, MGC10511 antibody, MAGEA11 antibody, "cancer/testis antigen family 1, member 11 antibody", MAGE-11 antigen antibody, cancer/testis antigen 1.11 antibody, melanoma-associated antigen 11 antibody, "melanoma antigen family A, 11 antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

48 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 78 and 173 of MAGEA11 (Uniprot ID# P43364)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAGEA11(107-10918) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft , using MAGEA11(107-10918) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:A431
B:H1299
10% SDS PAGE
107-10918 diluted at 1:5000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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