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Anti-MAP2K2 (S222) Antibody
Catalog #: 102-17730
Rabbit Anti-Human MAP2K2 (S222) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P36507 |
| Gene Id | 5605 |
| Gene Symbols | MAP2K2 |
| Protein Name / Synonyms | MAP2K2 (S222) |
| Clonality | Polyclonal |
| Preparation | This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2. |
| Concentration (lot specific) | 0.400 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken, Drosophila, Hamster, Rabbit, Xenopus
Other Names
MAP2K2; MEK2; MKK2; PRKMK2; Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAPK/ERK kinase 2
NCBI Accession #
NP_109587.1
Other Accession #
P36506; Q63932; Q90891; Q05116; Q01986; P29678; P31938; Q02750; Q63980; Q24324
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
44424
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of MAP2K2-S222 (Cat# 102-17730) in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222 (Cat# 102-17730). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) . |
 | Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) (Cat# 102-17730), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.