Anti-MAP3K9 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P80192
Gene Id	4293
Gene Symbols	MAP3K9
Protein Name / Synonyms	MAP3K9 (N-term)
Clonality	Polyclonal
Preparation	This MAP3K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the N-terminal region of human MAP3K9.
Concentration (lot specific)	0.490 mg/ml
Recommended Applications	IHC-P, Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Specificity

Predicted Reactivity

Mouse

Other Names

MAP3K9; MLK1; PRKE1; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1

NCBI Accession #

NP_149132.2

Other Accession #

Q3U1V8

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

121895

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	MAP3K9 Antibody (N-term) (Cat# 102-17731) western blot analysis in SW480 cell line lysates (35ug/lane).This demonstrates the MAP3K9 antibody detected the MAP3K9 protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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