Anti-MAPK1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P28482
Gene Id	5594
Gene Symbols	MAPK1
Protein Name / Synonyms	MAPK1 (C-term)
Clonality	Polyclonal
Preparation	This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-345 amino acids from the C-terminal region of human MAPK1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cardiovascular Disease, Cancer, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MAPK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2

NCBI Accession #

NP_002736.3;NP_620407.1

Other Accession #

P63086; P63085; P46196

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

41390

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	MAPK1 Antibody (C-term) (Cat# 102-17210) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow).
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAPK1 Antibody (C-term)(Cat# 102-17210), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	MAPK1 Antibody (C-term) (Cat# 102-17210) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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