3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MAPK1 (Center) Antibody
Catalog #: 102-17211
Rabbit Anti-Human MAPK1 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P28482 |
| Gene Id | 5594 |
| Gene Symbols | MAPK1 |
| Protein Name / Synonyms | MAPK1 (Center) |
| Clonality | Polyclonal |
| Preparation | This MAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the Central region of human MAPK1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cardiovascular Disease, Cancer, MAPK Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Xenopus
Other Names
MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; MAP kinase isoform p42; Mitogen-activated protein kinase 2
NCBI Accession #
NP_002736.3;NP_620407.1
Other Accession #
P26696; P63086; P63085; P46196
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
41390
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of anti-MAPK1 Antibody (Center) Pab (RB11609) in Hela, 293, and HepG2 cell line lysates. MAPK1 Antibody (Center)(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with MAPK1 Antibody (Center)(Cat# 102-17211), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | MAPK1 Antibody (Center) (Cat# 102-17211) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.