Anti-MAPK14 (Center T180/Y182) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q16539
Gene Id	1432
Gene Symbols	MAPK14
Protein Name / Synonyms	MAPK14 (Center T180/Y182)
Clonality	Polyclonal
Preparation	This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-192 amino acids from the Central region of human MAPK14.
Concentration (lot specific)	0.380 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Cancer, Inflammation, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish

Other Names

MAPK14; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; Cytokine suppressive anti-inflammatory drug-binding protein; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; Stress-activated protein kinase 2a

NCBI Accession #

NP_001306.1;NP_620581.1;NP_620582.1;NP_620583.1

Other Accession #

P70618; P47811; Q9DGE2; NP_001306.1; NP_620582.1; NP_620581.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

41293

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Fluorescent confocal image of A549 cell stained with MAPK14 Antibody (Center T180/Y182)(Cat# 102-11592).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAPK14 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.
	MAPK14 Antibody (Center T180/Y182) (Cat# 102-11592) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).
	MAPK14 Antibody (Center T180/Y182) (Cat# 102-11592)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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