Anti-MAPK15 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q8TD08
Gene Id	225689
Gene Symbols	MAPK15
Protein Name / Synonyms	MAPK15 (N-term)
Clonality	Polyclonal
Preparation	This MAPK15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human MAPK15.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	MAPK Signaling, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.

Specificity

Other Names

MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; Extracellular signal-regulated kinase 7; Extracellular signal-regulated kinase 8

NCBI Accession #

NP_620590.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

59832

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of ERK8 (arrow) using rabbit polyclonal ERK8 Antibody (N-term) (Cat# 102-17414). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK15 gene (Lane 2) (Origene Technologies).
	Western blot analysis of anti-ERK8 Antibody (N-term) (Cat# 102-17414) in T47D cell line lysates (35ug/lane). ERK8 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK8 antibody (N-term)(Cat# 102-17414), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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