Anti-MAPK3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Non-Human Primate (rhesus monkey)
Host Species	Rabbit
Accession Number	P27361
Gene Id	5595
Gene Symbols	MAPK3
Protein Name / Synonyms	MAPK3 (N-term)
Clonality	Polyclonal
Preparation	This MAPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MAPK3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cardiovascular Disease, Cancer, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2). ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.

Specificity

Other Names

MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; p44-ERK1

NCBI Accession #

NP_001035145.1;NP_001103361.1;NP_002737.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43136

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab (Cat# 102-17388).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK1 antibody (N-term) (Cat# 102-17388), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	MAPK3 Antibody (N-term) (Cat# 102-17388) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products