Anti-MAPK7 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13164
Gene Id	5598
Gene Symbols	MAPK7
Protein Name / Synonyms	MAPK7 (C-term)
Clonality	Polyclonal
Preparation	This MAPK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 776-805 amino acids from the C-terminal region of human MAPK7.
Concentration (lot specific)	1.200 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Inflammation, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

MAPK7; BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; Big MAP kinase 1; Extracellular signal-regulated kinase 5

NCBI Accession #

NP_002740.2;NP_620601.1;NP_620602.2;NP_620603.2

Other Accession #

P0C865; Q9WVS8; A5PKJ4

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

88386

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-ERK5 C-term Pab (Cat# 102-17391) in K562 cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human kideny carcinoma tissue reacted with ERK5 Antibody (C-term) (Cat# 102-17391), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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