Anti-MAPK8 (T183/Y185) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P45983
Gene Id	5599
Gene Symbols	MAPK8
Protein Name / Synonyms	MAPK8 (T183/Y185)
Clonality	Polyclonal
Preparation	This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	MAPK Signaling, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Specificity

Predicted Reactivity

Mouse, Rat, Xenopus

Other Names

MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1

NCBI Accession #

NP_001265476.1;NP_002741.1;NP_620634.1;NP_620637.1

Other Accession #

P49187; Q61831; P53779; Q8QHK8; P49185; Q91Y86; NP_620637

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

48296

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of lysate from Hela cell line, using JNK1 antibody(Cat# 102-10860). 102-10860 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	JNK1-T183/Y185 antibody (Cat# 102-10860) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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