Anti-MARK1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9P0L2
Gene Id	4139
Gene Symbols	MARK1
Protein Name / Synonyms	MARK1 (N-term)
Clonality	Polyclonal
Preparation	This MARK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the N-terminal region of human MARK1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MARK is a family of kinases that is known for its involvement in establishing cell polarity and in phosphorylating tau protein during Alzheimer neurodegeneration. Expression of MARK causes the phosphorylation of MAPs at their KXGS motifs, thereby detaching MAPs from the microtubules and thus facilitating the transport of particles. This occurs without impairing the intrinsic activity of motors because the velocity during active movement remains unchanged. In primary retinal ganglion cells, transfection with tau leads to the inhibition of axonal transport of mitochondria, APP vesicles, and other cell components which leads to starvation of axons and vulnerability against stress. This transport inhibition can be rescued by phosphorylating tau with MARK

Specificity

Other Names

MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c

NCBI Accession #

NP_061120.3

Other Accession #

Q2HIY1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

89003

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	The anti-MARK1 N-term Pab (Cat# 102-17148) is used in Western blot to detect MARK1 in P7 mouse whole brain lysate (60 ug). 1:250 dilution of anti-MARK1 pab was used. Data and protocol kindly provided by DR. Shengli Zhao, M.D., Ph.D. of Duke University Medical Center.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with MARK1 Antibody (N-term)(Cat# 102-17148), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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