Anti-MARK2 (EMK) (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q7KZI7
Gene Id	2011
Gene Symbols	MARK2
Protein Name / Synonyms	MARK2 (EMK) (C-term)
Clonality	Polyclonal
Preparation	This MARK2 (EMK) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-630 amino acids from the C-terminal region of human MARK2 (EMK).
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Specificity

Predicted Reactivity

Rat

Other Names

MARK2; EMK1; Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; MAP/microtubule affinity-regulating kinase 2; PAR1 homolog; PAR1 homolog b

NCBI Accession #

NP_001034558.2;NP_001156768.1;NP_001156769.1;NP_004945.4;NP_059672.2

Other Accession #

O08679; Q05512; Q15524

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

87911

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-EMK Antibody (C-term)(Cat# 102-17746) in mouse thymus tissue lysates (35ug/lane). EMK(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	EMK Antibody (V581) (Cat# 102-17746) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the EMK antibody detected the EMK protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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